Course-depth investigation inventory
This remains Grades 8-12 (semester / lab-skills course). It is not an AP authorization, college-credit promise, or certification claim. The workstream adds 8 source-linked cases, 8 labeled synthetic datasets, 10 numerical calibrations, and explicit image-based, virtual, model, and professionally supervised alternatives.
No improvised procedures, preservatives, latex or other allergens, or instruments are introduced by these public lessons. Hands-on work must follow the educator-approved procedure and SDS, use ethically and legally sourced preserved specimens, and respect opt-out access to an equivalent image-based, virtual, or model route without penalty. No live-animal procedures are part of this course.
Completion evidence across the course: defend a real specimen identification, explain a valid cross-specimen comparison, and state the limits of the evidence. Public worked answers are nonsecure practice; the Unit 08 percentage is a comparison tally, not morphology.
Unit 1: Approved route and safety defense [approved-routes]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 2, Science 3, Science 4, Science 5.
Sources: NSTA Scope on Safety member article teaser: Dissection--Don't cut out safety; NABT: The Use of Animals in Biology Education.
materials: Use the supplied safety-route-records records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain approved-routes, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: Which evidence route is approved before the unit begins, and what kind of evidence can it support?
prerequisites: Knows instrument names, PPE expectations, opt-out rights, and that no live-animal procedures are permitted.
variables: Route type, approval status, observed skill evidence, and conceptual evidence are recorded separately.
controls: Every route uses the same conceptual question and source standard; hands-on technique is never inferred from a paper response.
replication: Audit each route with the same checklist before instruction and again when evidence is submitted.
analysis: Calculate the share of routes with observed practical evidence and identify where a report would overclaim.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. Safety evidence also includes the chosen route, SDS/preservative/allergen check, legal and ethical source, disposal plan, and whether practical skill was observed.
Unit 2: Earthworm segmentation without ranking animals [earthworm-segmentation]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 2, Science 4, Science 5.
Sources: OpenStax Biology 2e: Animal Form and Function; OpenStax Biology 2e: Organizing Life on Earth.
materials: Use the supplied earthworm-segments records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain earthworm-segmentation, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: How does repeated segmentation help orient and identify the earthworm specimen?
prerequisites: Can orient a bilaterally symmetric specimen and record a measurement with units.
variables: Specimen length, visible segment count, orientation, and preservation condition.
controls: Count only visible rings under the approved procedure; do not infer missing rings from memory.
replication: Repeat the count after rotating the tray and compare with a partner or image-based route.
analysis: Compute visible segments per centimeter and describe why it is only local practice evidence.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. The student must identify a real earthworm structure and explain what segmentation does and does not prove.
Unit 3: Grasshopper appendages and arthropod variation [grasshopper-appendages]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 2, Science 4, Science 5.
Sources: OpenStax Biology 2e: Animal Form and Function; OpenStax Biology 2e: Organizing Life on Earth.
materials: Use the supplied grasshopper-appendage-lengths records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain grasshopper-appendages, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: Which grasshopper structures can be identified from direct evidence, and where does preservation limit the claim?
prerequisites: Knows arthropod regions, jointed appendages, and external orientation terms.
variables: Appendage pair, side, length, damage note, and identification confidence.
controls: Measure corresponding left-right positions only; do not compare different appendage types as if they were the same part.
replication: Have a second reader verify the side and damage note from the same specimen or image route.
analysis: Compute mean paired asymmetry and classify differences as matching, artifact-limited, or unresolved.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. The student must identify real grasshopper structures and state when variation or artifact limits the comparison.
Unit 4: Mollusk comparison and analogy limits [mollusk-comparison]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 3, Science 4, Science 5.
Sources: OpenStax Biology 2e: Understanding Evolution; Understanding Evolution: Homologies.
materials: Use the supplied mollusk-mantle-records records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain mollusk-comparison, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: Which mollusk comparisons are supported by visible anatomy, and which require outside phylogenetic evidence?
prerequisites: Can identify mantle, gills or siphon/funnel, and body orientation in the selected mollusk route.
variables: Specimen model, structure, function, comparison category, and evidence limit.
controls: Compare the same structural level; do not compare an organ function to a tissue origin as if they were the same claim.
replication: Repeat the classification with one clam/squid structure and one outside vertebrate example.
analysis: Compute the proportion practice and write one homologous, one analogous, and one unresolved comparison.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. The student must use homologous, analogous, and unresolved correctly in a mollusk comparison.
Unit 5: Perch vertebrate evidence without ancestor language [perch-vertebrate]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 2, Science 3, Science 4, Science 5.
Sources: OpenStax Biology 2e: Organizing Life on Earth; OpenStax Biology 2e: Animal Form and Function.
materials: Use the supplied perch-circulation-records records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain perch-vertebrate, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: What vertebrate features can be identified directly on the perch, and what evolutionary claims require outside evidence?
prerequisites: Can orient a fish specimen and distinguish gill, fin, heart, and vertebral-column evidence.
variables: Structure, chamber count, body length, gill count, preservation note, and comparison category.
controls: Do not use living-species order as ancestry order; separate direct observation from reference evidence.
replication: Compare two model records and repeat the claim with a tree diagram supplied by the educator.
analysis: Compute the caudal-to-body proportion and write a direct-observation claim plus a phylogenetic-evidence claim.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. The student must identify a real perch structure and state the evidence limit for fin-to-limb comparisons.
Unit 6: Frog systems and comparative physiology [frog-physiology]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 2, Science 3, Science 4, Science 5.
Sources: OpenStax Biology 2e: Animal Form and Function; OpenStax Biology 2e: Organizing Life on Earth.
materials: Use the supplied frog-system-records records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain frog-physiology, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: How do frog organ systems support comparative physiology while preserving evidence limits?
prerequisites: Can identify major thoracic and abdominal structures and read a simple quantitative record.
variables: Structure, measurement, chamber count, lung condition, preservation note, and comparison organism.
controls: Use the same measurement landmarks and label artifact before comparing physiology.
replication: Repeat the comparison with a second image or model when a preserved organ is collapsed or displaced.
analysis: Compute the ratio and write the physiological explanation plus the artifact warning.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. The student must defend a frog structure identification, a valid vertebrate comparison, and one preservation limit.
Unit 7: Fetal pig development and adult-body limits [fetal-pig-development]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 2, Science 3, Science 4, Science 5.
Sources: OpenStax Biology 2e: Animal Form and Function; OpenStax Biology 2e: Organizing Life on Earth.
materials: Use the supplied fetal-pig-proportions records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain fetal-pig-development, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: How does developmental stage change the evidence a fetal pig can and cannot provide?
prerequisites: Can identify mammalian organ systems and knows that fetal and adult physiology differ.
variables: Crown-rump length, organ measurement, fetal structure, preservation note, and comparison claim.
controls: Label fetal structures and preservation before comparing to adult or human references.
replication: Check the claim against a second specimen image or model and a reference diagram.
analysis: Compute the proportional measurement and write a fetal-versus-adult evidence limit.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. The student must defend fetal-pig identification with developmental context and state why the model is not a universal adult body.
Unit 8: Cross-specimen comparison defense [comparison-defense]
Inputs, synthetic data, selected-level tasks, and checked answers. Criteria: Science 1, Science 2, Science 3, Science 4, Science 5.
Sources: Understanding Evolution: Homologies; OpenStax Biology 2e: Understanding Evolution; OpenStax Biology 2e: Organizing Life on Earth.
materials: Use the supplied cross-specimen-evidence records, assigned source sections, calculator and an accessible graph or diagram. No physical apparatus is required.
record: Retain comparison-defense, the selected level, original inputs and first attempt, source/date, system and axes, units, assumptions, calculation/graph, uncertainty, correction and fresh transfer. Link only to the stated science criteria.
review: An educator reviews prerequisites, source interpretation, reasoning and accessibility before assessment. A paper response does not certify unobserved practical technique; approved hands-on work requires a separate record.
question: Which comparisons are homologous, analogous, or unresolved, and what evidence supports each category?
prerequisites: Can identify structures across at least two approved specimen routes and define homology and analogy.
variables: Specimens compared, visible evidence, outside evidence, classification, and limit.
controls: Compare the same structural level and keep specimen observation separate from phylogenetic reference evidence.
replication: Repeat the framework with a fresh comparison selected by the educator.
analysis: Tally the categories, then defend one unresolved case honestly.
Evidence: Completion evidence: the student defends a real specimen identification, explains a valid cross-specimen comparison, and states the limits of the evidence. The student must complete the dissection defense with a real identification, a valid comparison, and an evidence-limit statement.